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- Title
Reactive oxidant species in piriform cortex extracellular fluid during seizures induced by systemic kainic acid in rats.
- Authors
Layton, M E; Pazdernik, T L
- Abstract
Kainic acid (KA) administered systemically to rats produces seizures and brain damage. We measured an increase in reactive oxidant species (ROS) during KA-induced seizures in the extracellular fluid (ECF) of the piriform cortex, a brain region known to be subsequently damaged. Intracerebral microdialysis samples were collected and assayed for isoluminol-dependent chemiluminescence before and after injection of KA (16 mg/kg, i.p.). Hydrogen peroxide (H2O2) concentrations were calculated from catalase-sensitive chemiluminescence, the difference between total and catalase-resistant chemiluminescence. During generalized tonic-clonic seizures, both total and catalase-resistant chemiluminescence increased significantly in samples from brain ECF. Catalase-resistant chemiluminescence, most likely produced by ascorbic acid, increased for a full hour during sustained seizure activity. H2O2 concentrations showed a trend towards elevation during seizures. Increased ROS suggest that oxidative stress occurs in brain ECF during sustained seizure activity.
- Publication
Journal of molecular neuroscience : MN, 1999, Vol 13, Issue 1-2, p63
- ISSN
0895-8696
- Publication type
Journal Article
- DOI
10.1385/JMN:13:1-2:63