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- Title
A new inhibitor of apoptosis from vaccinia virus and eukaryotes.
- Authors
Gubser, Caroline; Bergamaschi, Daniele; Hollinshead, Michael; Lu, Xin; van Kuppeveld, Frank J M; Smith, Geoffrey L
- Abstract
A new apoptosis inhibitor is described from vaccinia virus, camelpox virus, and eukaryotic cells. The inhibitor is a hydrophobic, multiple transmembrane protein that is resident in the Golgi and is named GAAP (Golgi anti-apoptotic protein). Stable expression of both viral GAAP (v-GAAP) and human GAAP (h-GAAP), which is expressed in all human tissues tested, inhibited apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Conversely, knockout of h-GAAP by siRNA induced cell death by apoptosis. v-GAAP and h-GAAP display overlapping functions as shown by the ability of v-GAAP to complement for the loss of h-GAAP. Lastly, deletion of the v-GAAP gene from vaccinia virus did not affect virus replication in cell culture, but affected virus virulence in a murine infection model. This study identifies a new regulator of cell death that is highly conserved in evolution from plants to insects, amphibians, mammals, and poxviruses.
- Publication
PLoS pathogens, 2007, Vol 3, Issue 2, pe17
- ISSN
1553-7374
- Publication type
Journal Article
- DOI
10.1371/journal.ppat.0030017