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- Title
The role of HuR in the post-transcriptional regulation of interleukin-3 in T cells.
- Authors
González-Feliciano, José A; Hernández-Pérez, Marimar; Estrella, Luis A; Colón-López, Daisy D; López, Armando; Martínez, Marina; Maurás-Rivera, Kirla R; Lasalde, Clarivel; Martínez, Daviana; Araujo-Pérez, Félix; González, Carlos I
- Abstract
Human Interleukin-3 (IL-3) is a lymphokine member of a class of transiently expressed mRNAs harboring Adenosine/Uridine-Rich Elements (ARE) in their 3' untranslated regions (3'-UTRs). The regulatory effects of AREs are often mediated by specific ARE-binding proteins (ARE-BPs). In this report, we show that the human IL-3 3'-UTR plays a post-transcriptional regulation role in two human transformed cell lines. More specifically, we demonstrate that the hIL-3 3'-UTR represses the translation of a luciferase reporter both in HeLa and Jurkat T-cells. These results also revealed that the hIL-3 3'-UTR-mediated translational repression is exerted by an 83 nt region comprised mainly by AREs and some non-ARE sequences. Moreover, electrophoretic mobility shift assays (EMSAs) and UV-crosslinking analysis show that this hIL-3 ARE-rich region recruits five specific protein complexes, including the ARE-BPs HuR and TIA-1. HuR binding to this ARE-rich region appears to be spatially modulated during T-cell activation. Together, these results suggest that HuR recognizes the ARE-rich region and plays a role in the IL-3 3'-UTR-mediated post-transcriptional control in T-cells.
- Publication
PloS one, 2014, Vol 9, Issue 3, pe92457
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0092457