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- Title
Alterations in regulatory T cells induced by specific oligosaccharides improve vaccine responsiveness in mice.
- Authors
Schijf, Marcel A; Kerperien, Joann; Bastiaans, Jacqueline; Szklany, Kirsten; Meerding, Jenny; Hofman, Gerard; Boon, Louis; van Wijk, Femke; Garssen, Johan; Van't Land, Belinda
- Abstract
Prophylactic vaccinations are generally performed to protect naïve individuals with or without suppressed immune responsiveness. In a mouse model for Influenza vaccinations the specific alterations of CD4(+)CD25(+)Foxp3(+) regulatory T-cells (Tregs) in the immune modulation induced by orally supplied oligosaccharides containing scGOS/lcFOS/pAOS was assessed. This dietary intervention increased vaccine specific DTH responses. In addition, a significant increased percentage of T-bet(+) (Th1) activated CD69(+)CD4(+) T cells (p<0.001) and reduced percentage of Gata-3(+) (Th2) activated CD69(+)CD4(+)T cells (p<0.001) was detected in the mesenteric lymph nodes (MLN) of mice receiving scGOS/lcFOS/pAOS compared to control mice. Although no difference in the number or percentage of Tregs (CD4(+)Foxp3(+)) could be determined after scGOS/lcFOS/pAOS intervention, the percentage of CXCR3 (+) /T-bet(+) (Th1-Tregs) was significantly reduced (p<0.05) in mice receiving scGOS/lcFOS/pAOS as compared to mice receiving placebo diets. Moreover, although no absolute difference in suppressive capacity could be detected, an alteration in cytokine profile suggests a regulatory T cell shift towards a reducing Th1 suppression profile, supporting an improved vaccination response.
- Publication
PloS one, 2013, Vol 8, Issue 9, pe75148
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0075148