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- Title
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid.
- Authors
Parra, Gabriel I; Azure, Jolynn; Fischer, Renate; Bok, Karin; Sandoval-Jaime, Carlos; Sosnovtsev, Stanislav V; Sander, Peter; Green, Kim Y
- Abstract
Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52-56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses.
- Publication
PloS one, 2013, Vol 8, Issue 6, pe67592
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0067592