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- Title
A novel multiplex tetra-primer ARMS-PCR for the simultaneous genotyping of six single nucleotide polymorphisms associated with female cancers.
- Authors
Zhang, Chen; Liu, Ying; Ring, Brian Z; Nie, Kai; Yang, Mengjie; Wang, Miao; Shen, Hongwei; Wu, Xiyang; Ma, Xuejun
- Abstract
The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capillary electrophoresis (CE) for amplicon separation and identification. We assessed this process in the simultaneous genotyping of four breast cancer-and two cervical cancer risk-related SNPs.
- Publication
PloS one, 2013, Vol 8, Issue 4, pe62126
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0062126