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- Title
Protein Engineering with Biosynthesized Libraries from <i>Bordetella bronchiseptica</i> Bacteriophage.
- Authors
Yuan, Tom Z.; Overstreet, Cathie M.; Moody, Issa S.; Weiss, Gregory A.
- Abstract
Phage display offers a powerful approach to engineer protein affinity. A naturally occurring analog to phage display, the Bordetella bronchiseptica bacteriophage (BP) employs a highly variable protein termed the major tropism determinant (Mtd) to recognize its dynamic host. Propagation of BP provides a self-made phage library (SMPL) with vast numbers of phage particles, each displaying a single Mtd variant. We report applying the diversity of the BP-SMPL to access a tyrosine-rich library of Mtd variants. Expression of the SMPL-engineered Mtd variant as a GST-bound fusion protein demonstrated specific binding to the target T4 lysozyme with dissociation constants in the sub-micromolar range. The results guide future experiments with SMPLs applied to protein engineering.
- Publication
PLoS ONE, 2013, Vol 8, Issue 2, p1
- ISSN
1932-6203
- Publication type
Academic Journal
- DOI
10.1371/journal.pone.0055617