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- Title
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.
- Authors
Calaminus, Simon D J; Guitart, Amelie V; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P; Holyoake, Tessa L; Kranc, Kamil R; Machesky, Laura M
- Abstract
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.
- Publication
PloS one, 2012, Vol 7, Issue 12, pe51361
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0051361