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- Title
Host gene expression signatures discriminate between ferrets infected with genetically similar H1N1 strains.
- Authors
Ljungberg, Karl; McBrayer, Alexis; Camp, Jeremy V; Chu, Yong-Kyu; Tapp, Ronald; Noah, Diana L; Grimes, Sheila; Proctor, Mary L; Liljeström, Peter; Jonsson, Colleen B; Bruder, Carl E
- Abstract
Different respiratory viruses induce virus-specific gene expression in the host. Recent evidence, including those presented here, suggests that genetically related isolates of influenza virus induce strain-specific host gene regulation in several animal models. Here, we identified systemic strain-specific gene expression signatures in ferrets infected with pandemic influenza A/California/07/2009, A/Mexico/4482/2009 or seasonal influenza A/Brisbane/59/2007. Using uncorrelated shrunken centroid classification, we were able to accurately identify the infecting influenza strain with a combined gene expression profile of 10 selected genes, independent of the severity of disease. Another gene signature, consisting of 7 genes, could classify samples based on lung pathology. Furthermore, we identified a gene expression profile consisting of 31 probes that could classify samples based on both strain and severity of disease. Thus, we show that expression-based analysis of non-infected tissue enables distinction between genetically related influenza viruses as well as lung pathology. These results open for development of alternative tools for influenza diagnostics.
- Publication
PloS one, 2012, Vol 7, Issue 7, pe40743
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0040743