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- Title
A Two-Staged Model of Na<sup>+</sup> Exclusion in Rice Explained by 3D Modeling of HKT Transporters and Alternative Splicing.
- Authors
Cotsaftis, Olivier; Plett, Darren; Shirley, Neil; Tester, Mark; Hrmova, Maria
- Abstract
The HKT family of Na+ and Na+ /K+ transporters is implicated in plant salinity tolerance. Amongst these transporters, the cereal HKT1;4 and HKT1;5 are responsible for Na+ exclusion from photosynthetic tissues, a key mechanism for plant salinity tolerance. It has been suggested that Na+ is retrieved from the xylem transpiration stream either in the root or the leaf sheath, protecting the leaf blades from excessive Na+ accumulation. However, direct evidence for this scenario is scarce. Comparative modeling and evaluation of rice (Oryza sativa) HKT-transporters based on the recent crystal structure of the bacterial TrkH K+ transporter allowed to reconcile transcriptomic and physiological data. For OsHKT1;5, both transcript abundance and protein structural features within the selectivity filter could control shoot Na+ accumulation in a range of rice varieties. For OsHKT1;4, alternative splicing of transcript and the anatomical complexity of the sheath needed to be taken into account. Thus, Na+ accumulation in a specific leaf blade seems to be regulated by abundance of a correctly spliced OsHKT1;4 transcript in a corresponding sheath. Overall, allelic variation of leaf blade Na+ accumulation can be explained by a complex interplay of gene transcription, alternative splicing and protein structure.
- Publication
PLoS ONE, 2012, Vol 7, Issue 7, p1
- ISSN
1932-6203
- Publication type
Academic Journal
- DOI
10.1371/journal.pone.0039865