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- Title
Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
- Authors
Bordeaux, Jennifer M; Cheng, Huan; Welsh, Allison W; Haffty, Bruce G; Lannin, Donald R; Wu, Xingyong; Su, Nan; Ma, Xiao-Jun; Luo, Yuling; Rimm, David L
- Abstract
Quantification of mRNA has historically been done by reverse transcription polymerase chain reaction (RT-PCR). Recently, a robust method of detection of mRNA utilizing in situ hybridization has been described that is linear and shows high specificity with low background. Here we describe the use of the AQUA method of quantitative immunofluorescence (QIF) for measuring mRNA in situ using ESR1 (the estrogen receptor alpha gene) in breast cancer to determine its predictive value compared to Estrogen Receptor α (ER) protein.
- Publication
PloS one, 2012, Vol 7, Issue 5, pe36559
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0036559