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- Title
Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines.
- Authors
Powell, Ashley A; Talasaz, Amirali H; Zhang, Haiyu; Coram, Marc A; Reddy, Anupama; Deng, Glenn; Telli, Melinda L; Advani, Ranjana H; Carlson, Robert W; Mollick, Joseph A; Sheth, Shruti; Kurian, Allison W; Ford, James M; Stockdale, Frank E; Quake, Stephen R; Pease, R Fabian; Mindrinos, Michael N; Bhanot, Gyan; Dairkee, Shanaz H; Davis, Ronald W; Jeffrey, Stefanie S
- Abstract
To improve cancer therapy, it is critical to target metastasizing cells. Circulating tumor cells (CTCs) are rare cells found in the blood of patients with solid tumors and may play a key role in cancer dissemination. Uncovering CTC phenotypes offers a potential avenue to inform treatment. However, CTC transcriptional profiling is limited by leukocyte contamination; an approach to surmount this problem is single cell analysis. Here we demonstrate feasibility of performing high dimensional single CTC profiling, providing early insight into CTC heterogeneity and allowing comparisons to breast cancer cell lines widely used for drug discovery.
- Publication
PloS one, 2012, Vol 7, Issue 5, pe33788
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0033788