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- Title
Both TLR2 and TRIF contribute to interferon-β production during Listeria infection.
- Authors
Aubry, Camille; Corr, Sinéad C; Wienerroither, Sebastian; Goulard, Céline; Jones, Ruth; Jamieson, Amanda M; Decker, Thomas; O'Neill, Luke A J; Dussurget, Olivier; Cossart, Pascale
- Abstract
Synthesis of interferon-β (IFN-β) is an innate response to cytoplasmic infection with bacterial pathogens. Our recent studies showed that Listeria monocytogenes limits immune detection and IFN-β synthesis via deacetylation of its peptidoglycan, which renders the bacterium resistant to lysozyme degradation. Here, we examined signaling requirements for the massive IFN-β production resulting from the infection of murine macrophages with a mutant strain of L. monocytogenes, ΔpgdA, which is unable to modify its peptidoglycan. We report the identification of unconventional signaling pathways to the IFN-β gene, requiring TLR2 and bacterial internalization. Induction of IFN-β was independent of the Mal/TIRAP adaptor protein but required TRIF and the transcription factors IRF3 and IRF7. These pathways were stimulated to a lesser degree by wild-type L. monocytogenes. They operated in both resident and inflammatory macrophages derived from the peritoneal cavity, but not in bone marrow-derived macrophages. The novelty of our findings thus lies in the first description of TLR2 and TRIF as two critical components leading to the induction of the IFN-β gene and in uncovering that individual macrophage populations adopt different strategies to link pathogen recognition signals to IFN-β gene expression.
- Publication
PloS one, 2012, Vol 7, Issue 3, pe33299
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0033299