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- Title
A new single-step PCR assay for the detection of the zoonotic malaria parasite Plasmodium knowlesi.
- Authors
Lucchi, Naomi W; Poorak, Mitra; Oberstaller, Jenna; DeBarry, Jeremy; Srinivasamoorthy, Ganesh; Goldman, Ira; Xayavong, Maniphet; da Silva, Alexandre J; Peterson, David S; Barnwell, John W; Kissinger, Jessica; Udhayakumar, Venkatachalam
- Abstract
Recent studies in Southeast Asia have demonstrated substantial zoonotic transmission of Plasmodium knowlesi to humans. Microscopically, P. knowlesi exhibits several stage-dependent morphological similarities to P. malariae and P. falciparum. These similarities often lead to misdiagnosis of P. knowlesi as either P. malariae or P. falciparum and PCR-based molecular diagnostic tests are required to accurately detect P. knowlesi in humans. The most commonly used PCR test has been found to give false positive results, especially with a proportion of P. vivax isolates. To address the need for more sensitive and specific diagnostic tests for the accurate diagnosis of P. knowlesi, we report development of a new single-step PCR assay that uses novel genomic targets to accurately detect this infection.
- Publication
PloS one, 2012, Vol 7, Issue 2, pe31848
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0031848