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- Title
Accumulation of endogenous LITAF in aggresomes.
- Authors
Eaton, Heather E; Metcalf, Julie; Lacerda, Andressa Ferreira; Brunetti, Craig R
- Abstract
LITAF is a 161 amino acid cellular protein which includes a proline rich N-terminus and a conserved C-terminal domain known as the simple-like domain. Mutations in LITAF have been identified in Charcot-Marie tooth disease, a disease characterized by protein aggregates. Cells transfected with cellular LITAF reveal that LITAF is localized to late endosomes/lysosomes. Here we investigated the intracellular localization of endogenous LITAF. We demonstrated that endogenous LITAF accumulates at a discrete cytoplasmic site in BGMK cells that we identify as the aggresome. To determine the domain within LITAF that is responsible for the localization of LITAF to aggresomes, we created a construct that contained the C-terminal simple-like domain of LITAF and found that this construct also localizes to aggresomes. These data suggest the simple-like domain is responsible for targeting endogenous LITAF to the aggresome.
- Publication
PloS one, 2012, Vol 7, Issue 1, pe30003
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0030003