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- Title
APC/C<sup>Cdh1</sup>-Mediated Degradation of the F-Box Protein NIPA Is Regulated by Its Association with Skp1.
- Authors
von Klitzing, Christine; Huss, Richard; Illert, Anna Lena; Fröschl, Astrid; Wötzel, Sabine; Peschel, Christian; Bassermann, Florian; Duyster, Justus
- Abstract
NIPA (Nuclear Interaction Partner of Alk kinase) is an F-box like protein that targets nuclear Cyclin B1 for degradation. Integrity and therefore activity of the SCFNIPA E3 ligase is regulated by cell-cycle-dependent phosphorylation of NIPA, restricting substrate ubiquitination to interphase. Here we show that phosphorylated NIPA is degraded in late mitosis in an APC/CCdh1-dependent manner. Binding of the unphosphorylated form of NIPA to Skp1 interferes with binding to the APC/Cadaptor protein Cdh1 and therefore protects unphosphorylated NIPA from degradation in interphase. Our data thus define a novel mode of regulating APC/C-mediated ubiquitination.
- Publication
PLoS ONE, 2011, Vol 6, Issue 12, p1
- ISSN
1932-6203
- Publication type
Academic Journal
- DOI
10.1371/journal.pone.0028998