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- Title
A conserved GA element in TATA-less RNA polymerase II promoters.
- Authors
Seizl, Martin; Hartmann, Holger; Hoeg, Friederike; Kurth, Fabian; Martin, Dietmar E; Söding, Johannes; Cramer, Patrick
- Abstract
Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters.
- Publication
PloS one, 2011, Vol 6, Issue 11, pe27595
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0027595