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- Title
Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
- Authors
Annibale, Paolo; Vanni, Stefano; Scarselli, Marco; Rothlisberger, Ursula; Radenovic, Aleksandra
- Abstract
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
- Publication
PloS one, 2011, Vol 6, Issue 7, pe22678
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0022678