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- Title
Validation of endogenous control genes for gene expression studies on human ocular surface epithelium.
- Authors
Kulkarni, Bina; Mohammed, Imran; Hopkinson, Andrew; Dua, Harminder Singh
- Abstract
To evaluate a panel of ten known endogenous control genes (ECG) with quantitative reverse transcription PCR (qPCR), for identification of stably expressed endogenous control genes in the ocular surface (OS) epithelial regions including cornea, limbus, limbal epithelial crypt and conjunctiva to normalise the quantitative reverse transcription PCR data of genes of interest expressed in above-mentioned regions.
- Publication
PloS one, 2011, Vol 6, Issue 8, pe22301
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0022301