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- Title
A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.
- Authors
Cosnier, Bruno; Kwapisz, Marta; Hatin, Isabelle; Namy, Olivier; Hermann-Le Denmat, Sylvie; Morillon, Antonin; Rousset, Jean-Pierre; Fabret, Céline
- Abstract
In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a C-terminal elongated form of the Imp3 protein. A strain expressing the mutant allele displayed ribosome biogenesis defects equivalent to IMP3 depletion. This hypomorphic allele represented a unique opportunity to investigate and better understand the Imp3p functions. We demonstrated that the +1 frameshifting was increased in the mutant strain. Further characterizations revealed involvement of the Imp3 protein in DNA repair and telomere length control, pointing to a functional relationship between both pathways and ribosome biogenesis.
- Publication
PloS one, 2011, Vol 6, Issue 4, pe19500
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0019500