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- Title
New approach using the real-time PCR method for estimation of the toxic marine dinoflagellate Ostreopsis cf. ovata in marine environment.
- Authors
Perini, Federico; Casabianca, Anna; Battocchi, Cecilia; Accoroni, Stefano; Totti, Cecilia; Penna, Antonella
- Abstract
We describe the development and validation of a new quantitative real time PCR (qrt-PCR) method for the enumeration of the toxic benthic dinoflagellate Ostreopsis cf. ovata in marine environment. The benthic Ostreopsis sp. has a world-wide distribution and is associated during high biomass proliferation with the production of potent palytoxin-like compounds affecting human health and environment. Species-specific identification, which is relevant for the complex of different toxins production, by traditional methods of microscopy is difficult due to the high morphological variability, and thus different morphotypes can be easily misinterpreted.
- Publication
PloS one, 2011, Vol 6, Issue 3, pe17699
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0017699