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- Title
Sub-telomeric core X and Y' elements in S. cerevisiae suppress extreme variations in gene silencing.
- Authors
Power, Patricia; Jeffery, Daniel; Rehman, Muhammad Attiq; Chatterji, Arjun; Yankulov, Krassimir
- Abstract
Telomere Position Effect (TPE) is governed by strong repression signals emitted by telomeres via the Sir2/3/4 Histone Deacetylase complex. These signals are then relayed by weak proto-silencers residing in the subtelomeric core X and Y' elements. Subtelomeres also contain Sub-Telomeric Anti-silencing Regions (STARs). In this study we have prepared telomeres built of different combinations of core X, Y' and STARs and have analyzed them in strains lacking Histone-Acetyltransferase genes as well as in cdc6-1 and Δrif1 strains. We show that core X and Y' dramatically reduce both positive and negative variations in TPE, that are caused by these mutations. We also show that the deletion of Histone-Acetyltransferase genes reduce the silencing activity of an ACS proto-silencer, but also reduce the anti-silencing activity of a STAR. We postulate that core X and Y' act as epigenetic "cushioning" cis-elements.
- Publication
PloS one, 2011, Vol 6, Issue 3, pe17523
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0017523