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- Title
A mechanistic model of PCR for accurate quantification of quantitative PCR data.
- Authors
Boggy, Gregory J; Woolf, Peter J
- Abstract
Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle () standard curve quantification, which requires the time- and labor-intensive construction of a standard curve. In theory, the shape of a qPCR data curve can be used to directly quantify DNA concentration by fitting a model to data; however, current empirical model-based quantification methods are not as reliable as standard curve quantification.
- Publication
PloS one, 2010, Vol 5, Issue 8, pe12355
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0012355