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- Title
A flow cytometry-based FRET assay to identify and analyse protein-protein interactions in living cells.
- Authors
Banning, Carina; Votteler, Jörg; Hoffmann, Dirk; Koppensteiner, Herwig; Warmer, Martin; Reimer, Rudolph; Kirchhoff, Frank; Schubert, Ulrich; Hauber, Joachim; Schindler, Michael
- Abstract
Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a flow cytometry-based FRET assay and analysed interactions of human and simian immunodeficiency virus (HIV and SIV) Nef and Vpu proteins with cellular factors, as well as HIV Rev multimer-formation.
- Publication
PloS one, 2010, Vol 5, Issue 2, pe9344
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0009344