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- Title
Next-generation phage display: integrating and comparing available molecular tools to enable cost-effective high-throughput analysis.
- Authors
Dias-Neto, Emmanuel; Nunes, Diana N; Giordano, Ricardo J; Sun, Jessica; Botz, Gregory H; Yang, Kuan; Setubal, João C; Pasqualini, Renata; Arap, Wadih
- Abstract
Combinatorial phage display has been used in the last 20 years in the identification of protein-ligands and protein-protein interactions, uncovering relevant molecular recognition events. Rate-limiting steps of combinatorial phage display library selection are (i) the counting of transducing units and (ii) the sequencing of the encoded displayed ligands. Here, we adapted emerging genomic technologies to minimize such challenges.
- Publication
PloS one, 2009, Vol 4, Issue 12, pe8338
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0008338