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- Title
Design parameters to control synthetic gene expression in Escherichia coli.
- Authors
Welch, Mark; Govindarajan, Sridhar; Ness, Jon E; Villalobos, Alan; Gurney, Austin; Minshull, Jeremy; Gustafsson, Claes
- Abstract
Production of proteins as therapeutic agents, research reagents and molecular tools frequently depends on expression in heterologous hosts. Synthetic genes are increasingly used for protein production because sequence information is easier to obtain than the corresponding physical DNA. Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles.
- Publication
PloS one, 2009, Vol 4, Issue 9, pe7002
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0007002