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- Title
High-level production of amorpha-4,11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli.
- Authors
Tsuruta, Hiroko; Paddon, Christopher J; Eng, Diana; Lenihan, Jacob R; Horning, Tizita; Anthony, Larry C; Regentin, Rika; Keasling, Jay D; Renninger, Neil S; Newman, Jack D
- Abstract
Artemisinin derivatives are the key active ingredients in Artemisinin combination therapies (ACTs), the most effective therapies available for treatment of malaria. Because the raw material is extracted from plants with long growing seasons, artemisinin is often in short supply, and fermentation would be an attractive alternative production method to supplement the plant source. Previous work showed that high levels of amorpha-4,11-diene, an artemisinin precursor, can be made in Escherichia coli using a heterologous mevalonate pathway derived from yeast (Saccharomyces cerevisiae), though the reconstructed mevalonate pathway was limited at a particular enzymatic step.
- Publication
PloS one, 2009, Vol 4, Issue 2, pe4489
- ISSN
1932-6203
- Publication type
Journal Article
- DOI
10.1371/journal.pone.0004489