We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Integrating 5-hydroxymethylcytosine into the epigenomic landscape of human embryonic stem cells.
- Authors
Szulwach, Keith E; Li, Xuekun; Li, Yujing; Song, Chun-Xiao; Han, Ji Woong; Kim, SangSung; Namburi, Sandeep; Hermetz, Karen; Kim, Julie J; Rudd, M Katharine; Yoon, Young-Sup; Ren, Bing; He, Chuan; Jin, Peng
- Abstract
Covalent modification of DNA distinguishes cellular identities and is crucial for regulating the pluripotency and differentiation of embryonic stem (ES) cells. The recent demonstration that 5-methylcytosine (5-mC) may be further modified to 5-hydroxymethylcytosine (5-hmC) in ES cells has revealed a novel regulatory paradigm to modulate the epigenetic landscape of pluripotency. To understand the role of 5-hmC in the epigenomic landscape of pluripotent cells, here we profile the genome-wide 5-hmC distribution and correlate it with the genomic profiles of 11 diverse histone modifications and six transcription factors in human ES cells. By integrating genomic 5-hmC signals with maps of histone enrichment, we link particular pluripotency-associated chromatin contexts with 5-hmC. Intriguingly, through additional correlations with defined chromatin signatures at promoter and enhancer subtypes, we show distinct enrichment of 5-hmC at enhancers marked with H3K4me1 and H3K27ac. These results suggest potential role(s) for 5-hmC in the regulation of specific promoters and enhancers. In addition, our results provide a detailed epigenomic map of 5-hmC from which to pursue future functional studies on the diverse regulatory roles associated with 5-hmC.
- Publication
PLoS genetics, 2011, Vol 7, Issue 6, pe1002154
- ISSN
1553-7404
- Publication type
Journal Article
- DOI
10.1371/journal.pgen.1002154