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- Title
Identification of genes required for neural-specific glycosylation using functional genomics.
- Authors
Yamamoto-Hino, Miki; Kanie, Yoshimi; Awano, Wakae; Aoki-Kinoshita, Kiyoko F; Yano, Hiroyuki; Nishihara, Shoko; Okano, Hideyuki; Ueda, Ryu; Kanie, Osamu; Goto, Satoshi
- Abstract
Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, α1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific α1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating α1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.
- Publication
PLoS genetics, 2010, Vol 6, Issue 12, pe1001254
- ISSN
1553-7404
- Publication type
Journal Article
- DOI
10.1371/journal.pgen.1001254