Title

Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products.

Authors

Funane, Kazumi; Ishu, Tadashi; Terasawa, Kazue; Yamamoto, Tomoko; Kobayashi, Mikihiko

Abstract

Constructs six chimeric enzymes from two recombinant glucansucrases, dextransucrase S (DSRS) and dextransucrase 5 (DSRT5), by exchanging three substrate-binding regions in the N-terminal catalytic region. Effects of the regions on enzyme activity and the structures of the glucan products; Construction of chimeric DS genes; Preparation of glucansucrase chimeric enzymes.

Subjects

ENZYMES; BINDING sites; CATALYSTS; GLUCANS; GENES; PROTEINS

Publication

Bioscience, Biotechnology & Biochemistry, 2004, Vol 68, Issue 9, p1912

ISSN

0916-8451

Publication type

Academic Journal

DOI

10.1271/bbb.68.1912