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- Title
Quantitation of DNA methylation by melt curve analysis.
- Authors
Smith, Eric; Jones, Michael E; Drew, Paul A
- Abstract
Methylation of DNA is a common mechanism for silencing genes, and aberrant methylation is increasingly being implicated in many diseases such as cancer. There is a need for robust, inexpensive methods to quantitate methylation across a region containing a number of CpGs. We describe and validate a rapid, in-tube method to quantitate DNA methylation using the melt data obtained following amplification of bisulfite modified DNA in a real-time thermocycler.
- Publication
BMC cancer, 2009, Vol 9, p123
- ISSN
1471-2407
- Publication type
Journal Article
- DOI
10.1186/1471-2407-9-123