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- Title
Validation of oligoarrays for quantitative exploration of the transcriptome.
- Authors
Nygaard, Vigdis; Liu, Fang; Holden, Marit; Kuo, Winston P; Trimarchi, Jeff; Ohno-Machado, Lucila; Cepko, Connie L; Frigessi, Arnoldo; Glad, Ingrid K; Wiel, Mark A van de; Hovig, Eivind; Lyng, Heidi
- Abstract
Oligoarrays have become an accessible technique for exploring the transcriptome, but it is presently unclear how absolute transcript data from this technique compare to the data achieved with tag-based quantitative techniques, such as massively parallel signature sequencing (MPSS) and serial analysis of gene expression (SAGE). By use of the TransCount method we calculated absolute transcript concentrations from spotted oligoarray intensities, enabling direct comparisons with tag counts obtained with MPSS and SAGE. The tag counts were converted to number of transcripts per cell by assuming that the sum of all transcripts in a single cell was 5.105. Our aim was to investigate whether the less resource demanding and more widespread oligoarray technique could provide data that were correlated to and had the same absolute scale as those obtained with MPSS and SAGE.
- Publication
BMC genomics, 2008, Vol 9, p258
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-9-258