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- Title
Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISH.
- Authors
Wang, Kai; Guo, Wangzhen; Zhang, Tianzhen
- Abstract
Cotton, as an allopolyploid species, contains homoeologous A and D subgenomes. The study of the homoeologous (duplicated) segments or chromosomes can facilitate insight into the evolutionary process of polyploidy and the development of genomic resources. Fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones as probes has commonly been used to provide a reliable cytological technique for chromosome identification. In polyploids, it also presents a useful approach for identification and localization of duplicated segments. Here, two types of BACs that contained the duplicated segments were isolated and analyzed in tetraploid cotton by FISH.
- Publication
BMC genomics, 2007, Vol 8, p178
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-8-178