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- Title
A comprehensive platform for highly multiplexed mammalian functional genetic screens.
- Authors
Ketela, Troy; Heisler, Lawrence E; Brown, Kevin R; Ammar, Ron; Kasimer, Dahlia; Surendra, Anuradha; Ericson, Elke; Blakely, Kim; Karamboulas, Dina; Smith, Andrew M; Durbic, Tanja; Arnoldo, Anthony; Cheung-Ong, Kahlin; Koh, Judice L Y; Gopal, Shuba; Cowley, Glenn S; Yang, Xiaoping; Grenier, Jennifer K; Giaever, Guri; Root, David E; Moffat, Jason; Nislow, Corey
- Abstract
Genome-wide screening in human and mouse cells using RNA interference and open reading frame over-expression libraries is rapidly becoming a viable experimental approach for many research labs. There are a variety of gene expression modulation libraries commercially available, however, detailed and validated protocols as well as the reagents necessary for deconvolving genome-scale gene screens using these libraries are lacking. As a solution, we designed a comprehensive platform for highly multiplexed functional genetic screens in human, mouse and yeast cells using popular, commercially available gene modulation libraries. The Gene Modulation Array Platform (GMAP) is a single microarray-based detection solution for deconvolution of loss and gain-of-function pooled screens.
- Publication
BMC genomics, 2011, Vol 12, p213
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-12-213