We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
ChIP-chip versus ChIP-seq: lessons for experimental design and data analysis.
- Authors
Ho, Joshua W K; Bishop, Eric; Karchenko, Peter V; Nègre, Nicolas; White, Kevin P; Park, Peter J
- Abstract
Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone modifications. Previous reports only compared a small number of profiles, and little has been done to compare histone modification profiles generated by the two technologies or to assess the impact of input DNA libraries in ChIP-seq analysis. Here, we performed a systematic analysis of a modENCODE dataset consisting of 31 pairs of ChIP-chip/ChIP-seq profiles of the coactivator CBP, RNA polymerase II (RNA PolII), and six histone modifications across four developmental stages of Drosophila melanogaster.
- Publication
BMC genomics, 2011, Vol 12, p134
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-12-134