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- Title
A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening.
- Authors
You, Frank M; Luo, Ming-Cheng; Xu, Kenong; Deal, Karin R; Anderson, Olin D; Dvorak, Jan
- Abstract
A five-dimensional (5-D) clone pooling strategy for screening of bacterial artificial chromosome (BAC) clones with molecular markers utilizing highly-parallel Illumina GoldenGate assays and PCR facilitates high-throughput BAC clone and BAC contig anchoring on a genetic map. However, this strategy occasionally needs manual PCR to deconvolute pools and identify truly positive clones.
- Publication
BMC genomics, 2010, Vol 11, p692
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-11-692