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- Title
High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak.
- Authors
Gilmour, Matthew W; Graham, Morag; Van Domselaar, Gary; Tyler, Shaun; Kent, Heather; Trout-Yakel, Keri M; Larios, Oscar; Allen, Vanessa; Lee, Barbara; Nadon, Celine
- Abstract
A large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with Listeria monocytogenes serotype 1/2a occurred in Canada in 2008. Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct AscI PFGE patterns. High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic diversity associated with a change of a single restriction enzyme fragment during PFGE.
- Publication
BMC genomics, 2010, Vol 11, p120
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-11-120