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- Title
Sympatric ecological speciation meets pyrosequencing: sampling the transcriptome of the apple maggot Rhagoletis pomonella.
- Authors
Schwarz, Dietmar; Robertson, Hugh M; Feder, Jeffrey L; Varala, Kranthi; Hudson, Matthew E; Ragland, Gregory J; Hahn, Daniel A; Berlocher, Stewart H
- Abstract
The full power of modern genetics has been applied to the study of speciation in only a small handful of genetic model species--all of which speciated allopatrically. Here we report the first large expressed sequence tag (EST) study of a candidate for ecological sympatric speciation, the apple maggot Rhagoletis pomonella, using massively parallel pyrosequencing on the Roche 454-FLX platform. To maximize transcript diversity we created and sequenced separate libraries from larvae, pupae, adult heads, and headless adult bodies.
- Publication
BMC genomics, 2009, Vol 10, p633
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-10-633