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- Title
3' tag digital gene expression profiling of human brain and universal reference RNA using Illumina Genome Analyzer.
- Authors
Asmann, Yan W; Klee, Eric W; Thompson, E Aubrey; Perez, Edith A; Middha, Sumit; Oberg, Ann L; Therneau, Terry M; Smith, David I; Poland, Gregory A; Wieben, Eric D; Kocher, Jean-Pierre A
- Abstract
Massive parallel sequencing has the potential to replace microarrays as the method for transcriptome profiling. Currently there are two protocols: full-length RNA sequencing (RNA-SEQ) and 3'-tag digital gene expression (DGE). In this preliminary effort, we evaluated the 3' DGE approach using two reference RNA samples from the MicroArray Quality Control Consortium (MAQC).
- Publication
BMC genomics, 2009, Vol 10, p531
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-10-531