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- Title
A comparison of RNA amplification techniques at sub-nanogram input concentration.
- Authors
Lang, Julie E; Magbanua, Mark Jesus M; Scott, Janet H; Makrigiorgos, G Mike; Wang, Gang; Federman, Scot; Esserman, Laura J; Park, John W; Haqq, Christopher M
- Abstract
Gene expression profiling of small numbers of cells requires high-fidelity amplification of sub-nanogram amounts of RNA. Several methods for RNA amplification are available; however, there has been little consideration of the accuracy of these methods when working with very low-input quantities of RNA as is often required with rare clinical samples. Starting with 250 picograms-3.3 nanograms of total RNA, we compared two linear amplification methods 1) modified T7 and 2) Arcturus RiboAmp HS and a logarithmic amplification, 3) Balanced PCR. Microarray data from each amplification method were validated against quantitative real-time PCR (QPCR) for 37 genes.
- Publication
BMC genomics, 2009, Vol 10, p326
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-10-326