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- Title
A high resolution RH map of the bovine major histocompatibility complex.
- Authors
Brinkmeyer-Langford, Candice L; Childers, Christopher P; Fritz, Krista L; Gustafson-Seabury, Ashley L; Cothran, Marian; Raudsepp, Terje; Womack, James E; Skow, Loren C
- Abstract
The cattle MHC is termed the bovine leukocyte antigen (BoLA) and, along with the MHCs of other ruminants, is unique in its genomic organization. Consequently, correct and reliable gene maps and sequence information are critical to the study of the BoLA region. The bovine genome sequencing project has produced two assemblies (Btau_3.1 and 4.0) that differ substantially from each other and from conventional gene maps in the BoLA region. To independently compare the accuracies of the different sequence assemblies, we have generated a high resolution map of BoLA using a 12,000rad radiation hybrid panel. Seventy-seven unique sequence tagged site (STS) markers chosen at approximately 50 kb intervals from the Btau 2.0 assembly and spanning the IIa-III-I and IIb regions of the bovine MHC were mapped on a 12,000rad bovine radiation hybrid (RH) panel to evaluate the different assemblies of the bovine genome sequence.
- Publication
BMC genomics, 2009, Vol 10, p182
- ISSN
1471-2164
- Publication type
Journal Article
- DOI
10.1186/1471-2164-10-182