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- Title
A normalization strategy applied to HiCEP (an AFLP-based expression profiling) analysis: toward the strict alignment of valid fragments across electrophoretic patterns.
- Authors
Kadota, Koji; Fukumura, Ryutaro; Rodrigue, Joseph J; Araki, Ryoko; Abe, Masumi
- Abstract
Gene expression analysis based on comparison of electrophoretic patterns is strongly dependent on the accuracy of DNA fragment sizing. The current normalization strategy based on molecular weight markers has limited accuracy because marker peaks are often masked by intense peaks nearby. Cumulative errors in fragment lengths cause problems in the alignment of same-length fragments across different electropherograms, especially for small fragments (< 100 bp). For accurate comparison of electrophoretic patterns, further inspection and normalization of electrophoretic data after fragment sizing by conventional strategies is needed.
- Publication
BMC bioinformatics, 2005, Vol 6, p43
- ISSN
1471-2105
- Publication type
Journal Article
- DOI
10.1186/1471-2105-6-43