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- Title
Development and evaluation of new mask protocols for gene expression profiling in humans and chimpanzees.
- Authors
Toleno, Donna M; Renaud, Gabriel; Wolfsberg, Tyra G; Islam, Munirul; Wildman, Derek E; Siegmund, Kimberly D; Hacia, Joseph G
- Abstract
Cross-species gene expression analyses using oligonucleotide microarrays designed to evaluate a single species can provide spurious results due to mismatches between the interrogated transcriptome and arrayed probes. Based on the most recent human and chimpanzee genome assemblies, we developed updated and accessible probe masking methods that allow human Affymetrix oligonucleotide microarrays to be used for robust genome-wide expression analyses in both species. In this process, only data from oligonucleotide probes predicted to have robust hybridization sensitivity and specificity for both transcriptomes are retained for analysis.
- Publication
BMC bioinformatics, 2009, Vol 10, p77
- ISSN
1471-2105
- Publication type
Journal Article
- DOI
10.1186/1471-2105-10-77