We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling.
- Authors
Hong Wei Chu; Gally, Fabienne; Thaikoottathil, Jyoti; Janssen-Heininger, Yvonne M.; Qun Wu; Gongyi Zhang; Reisdorph, Nichole; Case, Stephanie; Minor, Maisha; Smith, Sean; Di Jiang; Michels, Nicole; Simon, Glenn; Martin, Richard J.
- Abstract
Background: Respiratory infections including Mycoplasma pneumoniae (Mp) contribute to various chronic lung diseases. We have shown that mouse short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein was able to inhibit Mp growth. Further, airway epithelial cells increased SPLUNC1 expression upon Mp infection. However, the mechanisms underlying SPLUNC1 regulation remain unknown. In the current study, we investigated if SPLUNC1 production following Mp infection is regulated through Toll-like receptor 2 (TLR2) signaling. Methods: Airway epithelial cell cultures were utilized to reveal the contribution of TLR2 signaling including NF-κB to SPLUNC1 production upon bacterial infection and TLR2 agonist stimulation. Results: Mp and TLR2 agonist Pam3CSK4 increased SPLUNC1 expression in tracheal epithelial cells from wild type, but not TLR2-/- BALB/c mice. RNA interference (short-hairpin RNA) of TLR2 in normal human bronchial epithelial cells under air-liquid interface cultures significantly reduced SPLUNC1 levels in Mp-infected or Pam3CSK4-treated cells. Inhibition and activation of NF-κB pathway decreased and increased SPLUNC1 production in airway epithelial cells, respectively. Conclusions: Our data for the first time suggest that airway epithelial TLR2 signaling is pivotal in mycoplasmainduced SPLUNC1 production, thus improving our understanding of the aberrant SPLUNC1 expression in airways of patients suffering from chronic lung diseases with bacterial infections.
- Publication
Respiratory Research, 2010, Vol 11, p155
- ISSN
1465-9921
- Publication type
Academic Journal
- DOI
10.1186/1465-9921-11-155