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- Title
MMP-9 activation by tumor trypsin-2 enhances in vivo invasion of human tongue carcinoma cells.
- Authors
Nyberg, P; Moilanen, M; Paju, A; Sarin, A; Stenman, U-H; Sorsa, T; Salo, T
- Abstract
Various human cancer cells express tumor-associated trypsinogen-2 (TAT-2), which can efficiently activate matrix metalloproteinases (MMPs) in vitro. MMP-2 and MMP-9 are particularly associated with the invasive malignant potential of several tumors. To investigate the role of TAT-2 in tumor invasion, we overexpressed TAT-2 in two malignant human squamous cell carcinoma cell lines of tongue and in non-malignant human papilloma virus transformed gingival keratinocytes. The TAT-2 overexpression significantly increased the levels of active MMP-9 in the most malignant cell line. TAT-2-transfected cells intravasated (invaded blood vessels) up to 60% more efficiently than did the control cells in an in vivo chick embryo chorioallantoic membrane invasion model. This increased intravasation was almost completely abolished by a specific tumor-associated trypsin inhibitor (TATI). These results indicate that TAT-2 has a role in the invasive growth of tumors, either alone or in cascade with gelatinases, especially by generating active MMP-9.
- Publication
Journal of dental research, 2002, Vol 81, Issue 12, p831
- ISSN
0022-0345
- Publication type
Journal Article
- DOI
10.1177/154405910208101207