Preparation and characterization of Jagged1-bound fibrinogen-based microspheres and their cytotoxicity against human dental pulp cells.

Manaspon, Chawan; Boonprakong, Lawan; Porntaveetus, Thantrira; Osathanon, Thanaphum

Surface immobilization of Jagged1 promotes odonto/osteogenic differentiation in human dental pulp cells. On the contrary, soluble Jagged1 fails to activate target gene expression of Notch signaling which is important for differentiation of human dental pulp cells. Hence, Jagged1 delivery system is indeed required for transportation of immobilized Jagged1 to promote odontogenic differentiation of human dental pulp cells in vivo. The present study described the preparation and characterization of Jagged1-bound fibrinogen-based microspheres. Water-in-oil emulsion technique was employed to prepare fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres. The average size of fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres was 213.9 ± 35.9 and 199.9 ± 41.9 µm, respectively. These microspheres did not alter the human dental pulp cells' cell viability. Human dental pulp cells were able to attach and spread on these microspheres. Jagged1 was conjugated on microspheres using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. Binding capacity of Jagged1 on both fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres ranged from 25.8 ± 6.0 to 35.6 ± 9.1%. There was no significant difference in the size of microspheres between before and after Jagged1 conjugation process. In conclusion, fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres could be utilized as the alternative biomaterials for Jagged1 delivery for future biomedical application.

DENTAL pulp; MICROSPHERES; NOTCH genes; CELL survival; GENE expression; IMMOBILIZED cells; BIOMATERIALS

Journal of Biomaterials Applications, 2020, Vol 34, Issue 8, p1105

0885-3282

Academic Journal

10.1177/0885328219898579