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- Title
HLA-DQ2 and -DQ8 signatures of gluten T cell epitopes in celiac disease.
- Authors
Tollefsen, Stig; Arentz-Hansen, Helene; Fleckenstein, Burkhard; Molberg, Oyvind; Ráki, Melinda; Kwok, William W; Jung, Günther; Lundin, Knut E A; Sollid, Ludvig M
- Abstract
Celiac disease is associated with HLA-DQ2 and, to a lesser extent, HLA-DQ8. Type 1 diabetes is associated with the same DQ molecules in the opposite order and with possible involvement of trans-encoded DQ heterodimers. T cells that are reactive with gluten peptides deamidated by transglutaminase 2 and invariably restricted by DQ2 or DQ8 can be isolated from celiac lesions. We used intestinal T cells from celiac patients to map DQ2 and DQ8 epitopes within 2 representative gluten proteins, alpha-gliadin AJ133612 and gamma-gliadin M36999. For alpha-gliadin, DQ2- and DQ8-restricted T cells recognized deamidated peptides of 2 separate regions. For gamma-gliadin, DQ2- and DQ8-restricted T cells recognized deamidated peptides of the same region. Some gamma-gliadin peptides were recognized by T cells in the context of DQ2 or DQ8 when bound in exactly the same registers, but with different requirements for deamidation; deamidation at peptide position 4 (P4) was important for DQ2-restricted T cells, whereas deamidation at P1 and/or P9 was important for DQ8-restricted T cells. Peptides combining the DQ2 and DQ8 signatures could be presented by DQ2, DQ8, and trans-encoded DQ heterodimers. Our findings shed light on the basis for the HLA associations in celiac disease and type 1 diabetes.
- Publication
The Journal of clinical investigation, 2006, Vol 116, Issue 8, p2226
- ISSN
0021-9738
- Publication type
Journal Article
- DOI
10.1172/JCI27620