We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of Next-Generation Sequencing Systems.
- Authors
Lin Liu; Yinhu Li; Siliang Li; Ni Hu; Yimin He; Ray Pong; Danni Lin; Lihua Lu; Maggie Law
- Abstract
With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries,make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world's biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized.
- Publication
Journal of Biomedicine & Biotechnology, 2012, Vol 2012, p1
- ISSN
1110-7243
- Publication type
Academic Journal
- DOI
10.1155/2012/251364