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- Title
Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization.
- Authors
Bunn, Jennifer A; Buford, Thomas W; Serra, Monica C; Kreider, Richard B; Willoughby, Darryn S
- Abstract
Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF-α, and NF-κB. Results. All genes of interest increased over time (P < .05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation.
- Publication
Journal of nutrition and metabolism, 2011, Vol 2011, p539690
- ISSN
2090-0732
- Publication type
Journal Article
- DOI
10.1155/2011/539690