We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Methodology Report Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies.
- Authors
Robison, Patrick; Hernández-Ochoa, Erick O.; Schneider, Martin F.
- Abstract
Primary culture models of single adult skeletal muscle fibers dissociated from locomotor muscles adhered to glass coverslips are routine and allow monitoring of functional processes in living cultured fibers. To date, such isolated fiber cultures have not been established for respiratory muscles, despite the fact that dysfunction of core respiratory muscles leading to respiratory arrest is the most common cause of death in many muscular diseases. Here we present the first description of an adherent culture system for single adult intercostal muscle fibers from the adult mouse. This system allows for monitoring functional properties of these living muscle fibers in culture with or without electrical field stimulation to drive muscle fiber contraction at physiological or pathological respiratory firing patterns. We also provide initial characterization of these fibers, demonstrating several common techniques in this new model system in the context of the established Flexor Digitorum Brevis muscle primary culture model.
- Publication
Journal of Biomedicine & Biotechnology, 2011, p1
- ISSN
1110-7243
- Publication type
Academic Journal
- DOI
10.1155/2011/393740